Unc13a^tm1Bros
Targeted Allele Detail

Summary

• Symbol: Unc13a^tm1Bros
• Name: unc-13 homolog A; targeted mutation 1, Nils Brose
• MGI ID: MGI:2449468
• Synonyms: Munc13-1-deficient, Unc13a^KO
• Gene: Unc13a Location: Chr8:72079356-72124418 bp, - strand Genetic Position: Chr8, 34.43 cM, cytoband B3.3
• Alliance: Unc13a^tm1Bros page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:56513
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The gene was disrupted by the replacement of 4 exons encoding amino acids 494-619 (which includes the C1 domain) with a neomycin resistance cassette via homologous recombination. The null allele was confirmed by Western blot analysis using antibodies directed against the extreme N- and C-termini of the protein. (J:56513)

Expression

In Structures Affected by this Mutation:

5 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Unc13a Mutation: 74 strains or lines available

References

• Original: J:56513 Augustin I, et al., Munc13-1 is essential for fusion competence of glutamatergic synaptic vesicles. Nature. 1999 Jul 29;400(6743):457-61
• All: 35 reference(s)