Btk^tm1Gsv
Targeted Allele Detail

Summary

• Symbol: Btk^tm1Gsv
• Name: Bruton agammaglobulinemia tyrosine kinase; targeted mutation 1, Frank Grosveld
• MGI ID: MGI:2449487
• Synonyms: Btk-
• Gene: Btk Location: ChrX:133443085-133484319 bp, - strand Genetic Position: ChrX, 56.18 cM
• Alliance: Btk^tm1Gsv page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:35769
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A vector was constructed that contained an in-frame fusion of 1.5 kb 5' genomic sequences of the targeted gene (up to exon 8) to the nuclear localization signal (nls) of the SV40 T antigen and the E. coli lacZ gene. After homologous recombination in ES cells, only exons 1 - 7 of the targeted gene were intact and were fused to the lacZ reporter. Western blots of total cell lysates from spleen cells and from thymocytes showed no detectable protein for the targeted gene in hemizygous mutant male mice. (J:35769)

Disease models

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Tumor Data

List all tumor models in MMHCdb carrying Btk^tm1Gsv

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Btk Mutation: 21 strains or lines available

References

• Original: J:35769 Hendriks RW, et al., Inactivation of Btk by insertion of lacZ reveals defects in B cell development only past the pre-B cell stage. EMBO J. 1996 Sep 16;15(18):4862-72
• All: 28 reference(s)