Prkg2^tm1Pfe
Targeted Allele Detail

Summary

• Symbol: Prkg2^tm1Pfe
• Name: protein kinase, cGMP-dependent, type II; targeted mutation 1, Alexander Pfeifer
• MGI ID: MGI:2449704
• Synonyms: cGKII^-, Prkg2^-
• Gene: Prkg2 Location: Chr5:99077632-99185042 bp, - strand Genetic Position: Chr5, 48.35 cM
• Alliance: Prkg2^tm1Pfe page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:37306
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A 308 bp fragment of the second coding exon was replaced with a neomycin selection cassette inserted by homologous recombination. The disrupted exon encoded a portion of the cGMP binding pocket. Northern blot, RT-PCR, and Western blot analyses indicated an absence of normal transcript and protein in homozygous mutant mice. Functional ablation was confirmed by a lack of cGMP-stimulated phosphotransferase activity in jejunal homogenates. (J:37306)

Expression

In Mice Carrying this Mutation:	13 assay results
In Structures Affected by this Mutation:	8 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Prkg2 Mutation: 42 strains or lines available

References

• Original: J:37306 Pfeifer A, et al., Intestinal secretory defects and dwarfism in mice lacking cGMP-dependent protein kinase II. Science. 1996 Dec 20;274(5295):2082-6
• All: 16 reference(s)