Slc1a2^tm1Kta
Targeted Allele Detail

Summary

• Symbol: Slc1a2^tm1Kta
• Name: solute carrier family 1 (glial high affinity glutamate transporter), member 2; targeted mutation 1, Kohichi Tanaka
• MGI ID: MGI:2449707
• Synonyms: EAAT2 KO, GLT-1^-
• Gene: Slc1a2 Location: Chr2:102489004-102621129 bp, + strand Genetic Position: Chr2, 54.13 cM
• Alliance: Slc1a2^tm1Kta page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:40984
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: The exon encoding the putative third transmembrane domain was disrupted by the insertion of a neomycin selection cassette. While transcript of approximately normal length was detected by Northern blot analysis of homozygous mutant cerebral RNA, RT-PCR analysis showed the transcript to lack exon 4. An absence of normal transcript was confirmed by in situ hybridization. Protein was undetected by Western blot analysis of cerebral membrane fractions. (J:40984)

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Slc1a2 Mutation: 40 strains or lines available

References

• Original: J:40984 Tanaka K, et al., Epilepsy and exacerbation of brain injury in mice lacking the glutamate transporter GLT-1. Science. 1997 Jun 13;276(5319):1699-702
• All: 34 reference(s)