Gli1^tm2Alj
Targeted Allele Detail

Summary

• Symbol: Gli1^tm2Alj
• Name: GLI-Kruppel family member GLI1; targeted mutation 2, Alexandra L Joyner
• MGI ID: MGI:2449767
• Synonyms: Gli1-LacZ, Gli1^LacZ, Gli1^lz, Gli1^lzki, Gli1^nlacZ, Gli1^null
• Gene: Gli1 Location: Chr10:127165751-127177448 bp, - strand Genetic Position: Chr10, 74.5 cM
• Alliance: Gli1^tm2Alj page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:79392
• Parent Cell Line: W4 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The gene was disrupted by replacement of a genomic region encoding the Gli N-terminal and zinc finger domains with a floxed-neo cassette and a lacZ having a nuclear localizing signal. The floxed-neo cassette was subsequently removed by crossing mutant animals to a cre deleter strain. The lacZ expression pattern in homozygous mutant embryos is identical to that of wild-type Gli as determined by immunohistochemical analysis. (J:79392)

Expression

In Mice Carrying this Mutation:

129 assay results

Tumor Data

List all tumor models in MMHCdb carrying Gli1^tm2Alj

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gli1 Mutation: 49 strains or lines available

References

• Original: J:79392 Bai CB, et al., Gli2, but not Gli1, is required for initial Shh signaling and ectopic activation of the Shh pathway. Development. 2002 Oct;129(20):4753-61
• All: 162 reference(s)