Dffb^tm1Osa
Targeted Allele Detail

Summary

• Symbol: Dffb^tm1Osa
• Name: DNA fragmentation factor, beta subunit; targeted mutation 1, Shigekazu Nagata
• MGI ID: MGI:2449797
• Synonyms: CAD^-
• Gene: Dffb Location: Chr4:154048904-154059578 bp, - strand Genetic Position: Chr4, 83.78 cM, cytoband E2
• Alliance: Dffb^tm1Osa page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:81474
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exons 1-6 and part of exon 7 were replaced with a neomycin resistance cassette via homologous recombination. Northern blot analysis of spleen and thymus RNA from homozygous mutant animals did not detect gene expression. (J:81474)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Dffb Mutation: 30 strains or lines available

References

• Original: J:81474 Kawane K, et al., Impaired thymic development in mouse embryos deficient in apoptotic DNA degradation. Nat Immunol. 2003 Feb;4(2):138-44
• All: 13 reference(s)