Dnase2a^tm1Osa
Targeted Allele Detail

Summary

• Symbol: Dnase2a^tm1Osa
• Name: deoxyribonuclease II alpha; targeted mutation 1, Shigekazu Nagata
• MGI ID: MGI:2449811
• Synonyms: Dnase II^-
• Gene: Dnase2a Location: Chr8:85635384-85638332 bp, + strand Genetic Position: Chr8, 41.31 cM
• Alliance: Dnase2a^tm1Osa page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:69831
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exons 1-5 and part of exon 6 were replaced with a neomycin resistance gene via homologous recombination. Absence of gene expression was confirmed by Northern blot analysis of fetal liver RNA from E14.5 homozygous mutant embryos. No enzyme activity could be detected in homozygous mutant fetal liver extracts. (J:69831)

Disease models

Expression

In Mice Carrying this Mutation:	72 assay results 1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Dnase2a Mutation: 93 strains or lines available

References

• Original: J:69831 Kawane K, et al., Requirement of DNase II for definitive erythropoiesis in the mouse fetal liver. Science. 2001 May 25;292(5521):1546-9
• All: 21 reference(s)