Pax6^tm1Lan
Targeted Allele Detail

Summary

• Symbol: Pax6^tm1Lan
• Name: paired box 6; targeted mutation 1, Richard A Lang
• MGI ID: MGI:2449854
• Synonyms: Pax6^deltaEE
• Gene: Pax6 Location: Chr2:105499245-105527709 bp, + strand Genetic Position: Chr2, 55.31 cM
• Alliance: Pax6^tm1Lan page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:73524
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Modified regulatory region, Null/knockout)
• Mutation: Disruption caused by insertion of vector
• Mutation details: The 341 bp ectoderm enhancer was deleted and replaced with a floxed neo cassette via homologous recombination. Transient expression of cre recombinase in correctly targeted cells removed the floxed neo cassette leaving behind a single loxP site in place of the ectoderm enhancer. PCR genotype analysis confirmed the mutant allele in homozygous animals. (J:73524)

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Pax6 Mutation: 94 strains or lines available

References

• Original: J:73524 Dimanlig PV, et al., The upstream ectoderm enhancer in Pax6 has an important role in lens induction. Development. 2001 Nov;128(22):4415-24
• All: 1 reference(s)