Ret^tm1Jmi
Targeted Allele Detail

Summary

• Symbol: Ret^tm1Jmi
• Name: ret proto-oncogene; targeted mutation 1, Jeffrey Milbrandt
• MGI ID: MGI:2449909
• Synonyms: Ret^GFP, Ret^TGM
• Gene: Ret Location: Chr6:118128706-118174679 bp, - strand Genetic Position: Chr6, 55.86 cM, cytoband E3-F1
• Alliance: Ret^tm1Jmi page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:71881
• Parent Cell Line: RW-4 (ES Cell)
• Strain of Origin: 129X1/SvJ

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutation: Insertion
• Mutation details: The gene was disrupted by insertion of an EGFP-derived cassette and a floxed neo cassette into exon 1 via homologous recombination. The floxed neo cassette was removed by mating mutant animals to cre deleter strain TgN(ACTB-cre)2Mrt. Immunohistochemical analysis of homozygous mutant newborns confirmed the absence of gene expression in spinal motorneurons. (J:71881)

Expression

In Mice Carrying this Mutation:	30 assay results
In Structures Affected by this Mutation:	4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ret Mutation: 54 strains or lines available

Notes

Homozygous mutant animals lacked kidneys and enteric neurons and died shortly after birth.

References

• Original: J:71881 Enomoto H, et al., RET signaling is essential for migration, axonal growth and axon guidance of developing sympathetic neurons. Development. 2001 Oct;128(20):3963-74
• All: 36 reference(s)