Hspg2^tm1Soin
Targeted Allele Detail

Summary

• Symbol: Hspg2^tm1Soin
• Name: perlecan (heparan sulfate proteoglycan 2); targeted mutation 1, Raija Soininen
• MGI ID: MGI:2449915
• Synonyms: Hspg2^delta3
• Gene: Hspg2 Location: Chr4:137196080-137297941 bp, + strand Genetic Position: Chr4, 69.93 cM
• Alliance: Hspg2^tm1Soin page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:81399
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exon 3 and flanking regions were deleted and replaced with a PGK-neo cassette via homologous recombination. The targeted mutation removes the heparan sulfate attachment while the core protein remains intact. RT-PC analysis of RNA from homozygous mutant embryos confirmed the exon 3 deletion. Fibroblasts derived from homozygous mutant cell culture were treated with heparitinase and chondroitinase, run on an SDS-PAGE gel, and immunoblotted to confirm the mutant protein retained the core protein and lacked heparan sulfate side chains. (J:81399)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Hspg2 Mutation: 310 strains or lines available

References

• Original: J:81399 Rossi M, et al., Heparan sulfate chains of perlecan are indispensable in the lens capsule but not in the kidney. EMBO J. 2003 Jan 15;22(2):236-45
• All: 13 reference(s)