Apc^tm3Mmt
Targeted Allele Detail

Summary

• Symbol: Apc^tm3Mmt
• Name: APC, WNT signaling pathway regulator; targeted mutation 3, Makoto M Taketo
• MGI ID: MGI:2449944
• Synonyms: Apc^neoR
• Gene: Apc Location: Chr18:34353977-34455605 bp, + strand Genetic Position: Chr18, 18.53 cM
• Alliance: Apc^tm3Mmt page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:81402
• Parent Cell Line: RW-4 (ES Cell)
• Strain of Origin: 129X1/SvJ

Mutation description

• Allele Type: Targeted (Conditional ready, Hypomorph)
• Mutation: Insertion
• Mutation details: LoxP sites flanking exons 11-13 were introduced and a neomycin resistance gene was inserted downstream of exon 13 in the direction opposite of transcription. RT-PCR analysis of heterozygous and homozygous mutant animals showed reduced gene expression at approximately 55% and 20% that of wild-type, respectively. This is a hypomorphic allele. (J:81402)

Expression

In Mice Carrying this Mutation:	29 assay results
In Structures Affected by this Mutation:	13 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Apc Mutation: 158 strains or lines available

References

• Original: J:81402 Ishikawa TO, et al., Requirement for tumor suppressor Apc in the morphogenesis of anterior and ventral mouse embryo. Dev Biol. 2003 Jan 15;253(2):230-46
• All: 3 reference(s)