Hprt1^{tm1(ACTB-Epor)Unc}
Targeted Allele Detail

Summary

• Symbol: Hprt1^{tm1(ACTB-Epor)Unc}
• Name: hypoxanthine phosphoribosyltransferase 1; targeted mutation 1, University of North Carolina
• MGI ID: MGI:2450106
• Synonyms: tEpoR
• Gene: Hprt1 Location: ChrX:52077014-52110536 bp, + strand Genetic Position: ChrX, 29.31 cM, cytoband A6
• Alliance: Hprt1^{tm1(ACTB-Epor)Unc} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:81859
• Parent Cell Line: E14TG2a (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Inserted expressed sequence)
• Mutations: Insertion, Intragenic deletion
• Hprt1^{tm1(ACTB-Epor)Unc} expresses 1 gene
  Knock-in expresses:
  

  Organism	Expressed Gene	Note
  mouse	Epor (MGI:95408)

• Mutation details: A targeting construct containing human beta actin promoter sequence adjoined to a cDNA fragment encoding a truncated erythropoietin receptor lacking 91 carboxy terminal amino acids was inserted at the endogenous locus via homologous recombination. An Hprt rescue technique using E14TG2a ES cells was used for selection to achieve a single copy insertion. (J:81859)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Hprt1 Mutation: 1279 strains or lines available

References

• Original: J:81859 Kirby SL, et al., Proliferation of multipotent hematopoietic cells controlled by a truncated erythropoietin receptor transgene. Proc Natl Acad Sci U S A. 1996 Sep 3;93(18):9402-7
• All: 2 reference(s)