Rcan1^tm1Eno
Targeted Allele Detail

Summary

• Symbol: Rcan1^tm1Eno
• Name: regulator of calcineurin 1; targeted mutation 1, Eric N Olson
• MGI ID: MGI:2450144
• Synonyms: MCIP1^-
• Gene: Rcan1 Location: Chr16:92188839-92263057 bp, - strand Genetic Position: Chr16, 53.6 cM
• Alliance: Rcan1^tm1Eno page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:81417
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exons 5 and 6 were replaced with a cassette containing the splice acceptor site of exon 5 fused to beta-galactosidase and neo. Alternative splicing of the targeted locus was expected to generate transcript encoding fusion proteins comprised of 29 amino terminal residues, encoded by either exon 1 or exon 4, followed by the beta galactosidase reporter. The putative fusion proteins were not expected to interact with or inhibit calcineurin. Normal transcript was undetected by Northern blot analysis of homozygous mutant heart tissue. Western blot analysis showed an absence of endogenous protein in homozygous mutant heart and brain tissue. Beta-galactosidase activity was detected and the expression pattern was similar to that of the endogenous gene. (J:81417)

Expression

In Mice Carrying this Mutation:	22 assay results
In Structures Affected by this Mutation:	1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rcan1 Mutation: 26 strains or lines available

References

• Original: J:81417 Vega RB, et al., Dual roles of modulatory calcineurin-interacting protein 1 in cardiac hypertrophy. Proc Natl Acad Sci U S A. 2003 Jan 21;100(2):669-74
• All: 8 reference(s)