Nfic^tm1Rmg
Targeted Allele Detail

Summary

• Symbol: Nfic^tm1Rmg
• Name: nuclear factor I/C; targeted mutation 1, Richard M Gronostajski
• MGI ID: MGI:2450153
• Synonyms: Nfic^-, NFI-C^-
• Gene: Nfic Location: Chr10:81232025-81267753 bp, - strand Genetic Position: Chr10, 39.72 cM, cytoband C1
• Alliance: Nfic^tm1Rmg page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:81645
• Parent Cell Line: E14.1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A 1.3 kb fragment encompassing exon 2 and flanking intronic sequence was replaced with a neomycin selection cassette inserted by homologous recombination. The deleted region encoded both the DNA-binding and dimerization domains. An aberrant transcript resulting from a splice of exon 1 to exon 3 was identified by RT-PCR analysis of homozygous mutant liver and brain tissues. Sequence analysis indicated that the mutant transcript is out of frame and terminates in exon 3, encoding a polypeptide comprised of 31 residues. (J:81645)

Expression

In Mice Carrying this Mutation:	2 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	9 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Nfic Mutation: 36 strains or lines available

References

• Original: J:81645 Steele-Perkins G, et al., Essential role for NFI-C/CTF transcription-replication factor in tooth root development. Mol Cell Biol. 2003 Feb;23(3):1075-84
• All: 12 reference(s)