Hnf1a^tm2Ylee
Targeted Allele Detail

Summary

• Symbol: Hnf1a^tm2Ylee
• Name: HNF1 homeobox A; targeted mutation 2, Ying-Hue Lee
• MGI ID: MGI:2450154
• Synonyms: Hnf-1alpha^kin
• Gene: Hnf1a Location: Chr5:115087039-115109121 bp, - strand Genetic Position: Chr5, 55.99 cM, cytoband F
• Alliance: Hnf1a^tm2Ylee page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:81639
• Parent Cell Line: RW-4 (ES Cell)
• Strain of Origin: 129X1/SvJ

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: A floxed PGK-neo cassette was inserted into intron 1 by homologous recombination. Transcript was undetected by Northern blot analysis of homozygous mutant liver tissue. The rescue of the allele was demonstrated by tissue-specific expression of cre. Northern blot analysis verified the presence of transcript in the livers of in rescued mice. (J:81639)

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Hnf1a Mutation: 29 strains or lines available

References

• Original: J:81639 Lee YH, et al., Liver-specific reactivation of the inactivated Hnf-1alpha gene: elimination of liver dysfunction to establish a mouse MODY3 model. Mol Cell Biol. 2003 Feb;23(3):923-32
• All: 2 reference(s)