Mtnr1a^tm1Rep
Targeted Allele Detail

Summary

• Symbol: Mtnr1a^tm1Rep
• Name: melatonin receptor 1A; targeted mutation 1, Steven M Reppert
• MGI ID: MGI:2450160
• Synonyms: MT1^-, MT1 KO
• Gene: Mtnr1a Location: Chr8:45522174-45541543 bp, + strand Genetic Position: Chr8, 24.95 cM
• Alliance: Mtnr1a^tm1Rep page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:41859
• Parent Cell Line: J1 (ES Cell)
• Strain of Origin: 129S4/SvJae

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exon 1 was replaced with a neomycin selection cassette inserted by homologous recombination. The deleted region encoded the 5' untranslated region and the first cytoplasmic loop. Autoradiography showed an absence of melatonin binding in sections of homozygous mutant brains, indicating complete functional ablation. (J:41859)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Mtnr1a Mutation: 25 strains or lines available

References

• Original: J:41859 Liu C, et al., Molecular dissection of two distinct actions of melatonin on the suprachiasmatic circadian clock. Neuron. 1997 Jul;19(1):91-102
• All: 29 reference(s)