S100a9^tm1Nck
Targeted Allele Detail

Summary

• Symbol: S100a9^tm1Nck
• Name: S100 calcium binding protein A9 (calgranulin B); targeted mutation 1, Wolfgang Nacken
• MGI ID: MGI:2450165
• Synonyms: Mrp14^-
• Gene: S100a9 Location: Chr3:90599939-90603028 bp, - strand Genetic Position: Chr3, 39.91 cM, cytoband F1-F2
• Alliance: S100a9^tm1Nck page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:81642
• Parent Cell Line: E14.1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• S100a9^tm1Nck involves 1 genes/genome features (S100a8) View all
• Mutation details: A 769 bp fragment encompassing exon 2 and flanking intronic sequence was replaced with a neomycin selection cassette inserted by homologous recombination. The translational start codon was included in the deleted region. Transcript was undetected by Northern blot and RT-PCR analyses of various tissues obtained from homozygous mutant mice. The absence of protein was indicated by flow cytometric, Western blot, and immunostaining analyses. S100A and S100A8 proteins are not detected in peripheral leukocytes. (J:81642)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any S100a9 Mutation: 18 strains or lines available

References

• Original: J:81642 Manitz MP, et al., Loss of S100A9 (MRP14) results in reduced interleukin-8-induced CD11b surface expression, a polarized microfilament system, and diminished responsiveness to chemoattractants in vitro. Mol Cell Biol. 2003 Feb;23(3):1034-43
• All: 41 reference(s)