Wnt3^tm2Brd
Targeted Allele Detail

Summary

• Symbol: Wnt3^tm2Brd
• Name: wingless-type MMTV integration site family, member 3; targeted mutation 2, Allan Bradley
• MGI ID: MGI:2450289
• Synonyms: Wnt3^Brdm2, Wnt3^{In(11Trp53;11Wnt3)8Brd}
• Gene: Wnt3 Location: Chr11:103664976-103708783 bp, + strand Genetic Position: Chr11, 67.5 cM
• Alliance: Wnt3^tm2Brd page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:56548
• Parent Cell Line: AB2.2 (ES Cell)
• Strain of Origin: 129S7/SvEvBrd-Hprt1^b-m2

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Wnt3^tm2Brd involves 1 genes/genome features (Wnt3) View all
• Mutation details: A 2.1-kb genomic DNA fragment containing exon 3 and most of exon 4 were replaced with a cassette, oriented toward the centromere, containing a neomycin resistance gene, a loxP site, the 5' half of an Hprt minigene, and a Krt14-Agouti coat color transgene. (J:56548)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 3 strains available Cell Lines: 0 lines available
• Carrying any Wnt3 Mutation: 25 strains or lines available

Notes

Oppositely-oriented loxP sites in Wnt3^tm2Brd and Trp53^tm2Brd, which was generated on the same Chr 11 homolog (in cis) in ES cells already carrying the Wnt3 mutation, served as the proximal and distal breakpoints, respectively, of a 24-cM engineered inversion, In(11Trp53;11Wnt3)8Brd. (J:56548)

References

• Original: J:56548 Zheng B, et al., Engineering a mouse balancer chromosome. Nat Genet. 1999 Aug;22(4):375-8
• All: 2 reference(s)