Plekha5^{Tg(AMH-cre)1Flor}
Transgenic Allele Detail

Summary

• Symbol: Plekha5^{Tg(AMH-cre)1Flor}
• Name: pleckstrin homology domain containing, family A member 5; transgene insertion 1, Florian Guillou
• MGI ID: MGI:2450300
• Synonyms: AMH-Cre, Mis-Cre, Tg(AMH-cre)1Flor
• Gene: Plekha5 Location: Chr6:140369780-140542836 bp, + strand Genetic Position: Chr6, 70.34 cM
• Alliance: Plekha5^{Tg(AMH-cre)1Flor} page

Loss of germ cells in adult Cyp26b1^tm1Ptk/Cyp26b1^tm1Ptk Plekha5^{Tg(AMH-cre)1Flor}/0 animals

Show the 4 phenotype image(s) involving this allele.

Transgene origin

• Strain of Origin: (C57BL/6 x SJL)F1

Transgene description

• Transgene Type: Transgenic (No functional change, Recombinase)
• Mutation: Insertion
• Plekha5^{Tg(AMH-cre)1Flor} expression driven by 1 gene
  Transgene expression driven by:
  

  Organism

  Driver Gene

  Homolog in Mouse

  Note

  human

  AMH (268)

  Amh	(MGI:88006)

• Mutation details: The transgene consists of a human AMH promoter, a cre recombinase gene, and genomic sequences from MT1 to provide mRNA secondary structure stability. The gene trap inserted into intron 3. Northern blot analysis shows gonad-specific transgene expression in Sertoli cells of 6 day old male mice and granulosa cells of 16 day old female mice. RT-PCR confirmed normal endogenous gene expression. The insertion site of the original cre transgene was subsequenly identified as intron 3 of Plekha5. (J:81650, J:189585)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	AMH (human) Summary of all recombinase alleles driven by AMH. Comparative matrix view of recombinase activities Alliance human AMH gene page

Expression

In Mice Carrying this Mutation:

192 assay results 13 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Plekha5 Mutation: 109 strains or lines available

References

• Original: J:81650 Lecureuil C, et al., Sertoli and granulosa cell-specific Cre recombinase activity in transgenic mice. Genesis. 2002 Jul;33(3):114-8
• All: 130 reference(s)