Mfn1^tm1Dcc
Targeted Allele Detail

Summary

• Symbol: Mfn1^tm1Dcc
• Name: mitofusin 1; targeted mutation 1, David C Chan
• MGI ID: MGI:2450304
• Synonyms: Mfn1
• Gene: Mfn1 Location: Chr3:32583614-32633388 bp, + strand Genetic Position: Chr3, 15.75 cM, cytoband B
• Alliance: Mfn1^tm1Dcc page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:81438
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129S/SvEv

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exon 3 of the endogenous allele was disrupted by the insertion of a neomycin selection cassette via homologous recombination. The deleted region encoded the G1 and G2 motifs of the GTPase domain. The insertion introduced a translational stop codon into the 5' region of exon 3. Protein was undetected by Western blot analysis of homozygous mutant embryos. (J:81438)

Expression

In Mice Carrying this Mutation:	1 assay results
In Structures Affected by this Mutation:	1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Mfn1 Mutation: 45 strains or lines available

References

• Original: J:81438 Chen H, et al., Mitofusins Mfn1 and Mfn2 coordinately regulate mitochondrial fusion and are essential for embryonic development. J Cell Biol. 2003 Jan 21;160(2):189-200
• All: 12 reference(s)