Mfn2^tm1Dcc
Targeted Allele Detail

Summary

• Symbol: Mfn2^tm1Dcc
• Name: mitofusin 2; targeted mutation 1, David C Chan
• MGI ID: MGI:2450306
• Synonyms: Mfn2^-
• Gene: Mfn2 Location: Chr4:147958056-147989161 bp, - strand Genetic Position: Chr4, 78.56 cM
• Alliance: Mfn2^tm1Dcc page

Defective giant cell layer of Mfn2^tm1Dcc/Mfn2^tm1Dcc placenta

Show the 1 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:81438
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129S/SvEv

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exon 3 of the endogenous allele was disrupted by the insertion of a neomycin selection cassette via homologous recombination. The deleted region encoded the G1 and G2 motifs of the GTPase domain. The insertion introduced a translational stop codon into the 5' region of exon 3. Protein was undetected by Western blot analysis of homozygous mutant embryos. (J:81438)

Expression

In Mice Carrying this Mutation:	2 assay results
In Structures Affected by this Mutation:	3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Mfn2 Mutation: 27 strains or lines available

References

• Original: J:81438 Chen H, et al., Mitofusins Mfn1 and Mfn2 coordinately regulate mitochondrial fusion and are essential for embryonic development. J Cell Biol. 2003 Jan 21;160(2):189-200
• All: 13 reference(s)