Chat^tm1Fhg
Targeted Allele Detail

Summary

• Symbol: Chat^tm1Fhg
• Name: choline O-acetyltransferase; targeted mutation 1, Fred H Gage
• MGI ID: MGI:2450310
• Synonyms: ChAT(-)
• Gene: Chat Location: Chr14:32130160-32187866 bp, - strand Genetic Position: Chr14, 19.4 cM
• Alliance: Chat^tm1Fhg page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:81738
• Parent Cell Line: J1 (ES Cell)
• Strain of Origin: 129S4/SvJae

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exons 11, 12, and 13 were replaced with a neomycin selection cassette inserted by homologous recombination. Normal protein was undetected by immunostaining of the basal forebrain of homozygous mutant embryos. Assays of the brain stem, spinal cord, and septum showed an absence of endogenous activity. (J:81738)
• Inheritance: Recessive

Disease models

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Chat Mutation: 58 strains or lines available

References

• Original: J:81738 Brandon EP, et al., Aberrant patterning of neuromuscular synapses in choline acetyltransferase-deficient mice. J Neurosci. 2003 Jan 15;23(2):539-49
• All: 11 reference(s)