Ros1^tm1Cbm
Targeted Allele Detail

Summary

• Symbol: Ros1^tm1Cbm
• Name: Ros1 proto-oncogene; targeted mutation 1, Carmen Birchmeier
• MGI ID: MGI:2450566
• Synonyms: c-ros-, ros^ex
• Gene: Ros1 Location: Chr10:51921817-52071340 bp, - strand Genetic Position: Chr10, 26.18 cM
• Alliance: Ros1^tm1Cbm page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:33259
• Parent Cell Line: E14.1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: A neomycin resistance cassette containing stop codons in all three reading frames was inserted into an exon that encodes the extracellular domain. This region corresponds to nucleotides 2962-3370 of the cDNA. Northern blot analyses of intestine from homozygous mutant mice detected two neo fusion transcripts using a cDNA probe for extracellular sequences and did not detect wild-type transcript. A cDNA probe for kinase sequences detected only one of the fusion transcripts. Northern blot analysis of testis dissected from homozygous mutant embryos detected wild-type transcript using a cDNA probe for kinase sequences. Western blot analysis using antibodies against a carboxy-terminal peptide did not detect protein in epididymal extracts from homozygous mutant mice. (J:33259)

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ros1 Mutation: 145 strains or lines available

References

• Original: J:33259 Sonnenberg-Riethmacher E, et al., The c-ros tyrosine kinase receptor controls regionalization and differentiation of epithelial cells in the epididymis. Genes Dev. 1996 May 15;10(10):1184-93
• All: 10 reference(s)