Tg(CD4-HIV)F26622Pjo
Transgene Detail

Summary

• Symbol: Tg(CD4-HIV)F26622Pjo
• Name: transgene insertion F26622, Paul Jolicoeur
• MGI ID: MGI:2450567
• Synonyms: CD4C/HIV^MutB
• Transgene: Tg(CD4-HIV)F26622Pjo Location: unknown
• Alliance: Tg(CD4-HIV)F26622Pjo page

Transgene origin

• Strain of Origin: (C57BL/6 x C3H)F2

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Mutation details: Mouse CD4 enhancer sequences, the human CD4 promoter, 8.8 kb of HIV sequences, and an SV40 polyadenylation signal was used for the transgene. The enhancer and promoter sequences were used to drive expression in CD4+ T cells and cells of the dendritic/macrophage lineage. The HIV sequence contained a stop oligo insertion at nucleotide 6400 of the env gene. Northern blot analysis showed high transgene expression in the thymus, moderate expression in lymph nodes, and weak expression in kidneys, lungs, intestines, and liver. (J:78756)

Expression

In Structures Affected by this Mutation:

5 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

Notes

Transgenic founder lines F26132, F26605, and F26622 were established. The latency of disease was reflective of the level of transgene expression. Founders F26605 and F26622 developed a disease phenotype similar to that observed in MutA trangenics. The high-expressing founder F26605 died early at less than 2 months of age, whereas moderate-expressing founder F26622 died at a later time. Low-expressing founder F26132 did not exhibit a phenotype.

References

• Original: J:78756 Hanna Z, et al., Nef harbors a major determinant of pathogenicity for an AIDS-like disease induced by HIV-1 in transgenic mice. Cell. 1998 Oct 16;95(2):163-75
• All: 2 reference(s)