Rag2^tm1Mao
Targeted Allele Detail

Summary

• Symbol: Rag2^tm1Mao
• Name: recombination activating gene 2; targeted mutation 1, Marjorie A Oettinger
• MGI ID: MGI:2450735
• Synonyms: c
• Gene: Rag2 Location: Chr2:101455063-101462874 bp, + strand Genetic Position: Chr2, 53.87 cM
• Alliance: Rag2^tm1Mao page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:81837
• Parent Cell Line: TC1/TC-1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted
• Mutations: Insertion, Intragenic deletion
• Mutation details: The endogenous locus was replaced with a floxed neo cassette followed by cDNA sequence encoding a mutant truncated RAG2 protein ('core' RAG2) lacking the C-terminal 144 amino acids. The floxed neo cassette was subsequently removed by transient expression of cre recombinase in correctly targeted cells. (J:81837)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rag2 Mutation: 119 strains or lines available

References

• Original: J:81837 Akamatsu Y, et al., Deletion of the RAG2 C terminus leads to impaired lymphoid development in mice. Proc Natl Acad Sci U S A. 2003 Feb 4;100(3):1209-14
• All: 1 reference(s)