Cacna1e^tm1Hssh
Targeted Allele Detail

Summary

• Symbol: Cacna1e^tm1Hssh
• Name: calcium channel, voltage-dependent, R type, alpha 1E subunit; targeted mutation 1, Hee-Sup Shin
• MGI ID: MGI:2451031
• Synonyms: alpha1E^-
• Gene: Cacna1e Location: Chr1:154266477-154760247 bp, - strand Genetic Position: Chr1, 66.14 cM
• Alliance: Cacna1e^tm1Hssh page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:81788
• Parent Cell Line: J1 (ES Cell)
• Strain of Origin: 129S4/SvJae

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The gene was disrupted by replacement of nucleotides 357 - 860 of exon 1 with a GFP-neo cassette via homologous recombination. The gene targeting event results in deletion of the translation initiation site and part of the N-terminal cytosolic domain. Absence of gene expression in homozygous mutant animals was confirmed by Western blot analysis of brain proteins. (J:81788)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cacna1e Mutation: 102 strains or lines available

References

• Original: J:81788 Lee SC, et al., Molecular basis of R-type calcium channels in central amygdala neurons of the mouse. Proc Natl Acad Sci U S A. 2002 Mar 5;99(5):3276-81
• All: 2 reference(s)