Slc15a2^tm1Dsmi
Targeted Allele Detail

Summary

• Symbol: Slc15a2^tm1Dsmi
• Name: solute carrier family 15 (H+/peptide transporter), member 2; targeted mutation 1, David E Smith
• MGI ID: MGI:2451265
• Synonyms: Pept2^-
• Gene: Slc15a2 Location: Chr16:36570539-36605324 bp, - strand Genetic Position: Chr16, 25.97 cM
• Alliance: Slc15a2^tm1Dsmi page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:81906
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A fragment encompassing a portion of exon 1 and all of exons 2 and 3 was replaced by a lacZ-neo cassette inserted by homologous recombination. The deleted region encoded the first 112 amino acids. Transcript was undetected in homozygous mutant mice by Northern blot analysis of renal RNA and RT-PCR analysis of choroid plexus RNA. Western blot analysis indicated an absence of encoded protein in homozygous mutant kidney brush border vesicle preparations. (J:81906)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Slc15a2 Mutation: 65 strains or lines available

References

• Original: J:81906 Shen H, et al., Targeted disruption of the PEPT2 gene markedly reduces dipeptide uptake in choroid plexus. J Biol Chem. 2003 Feb 14;278(7):4786-91
• All: 10 reference(s)