Rr27^tm1Tilg
Targeted Allele Detail

Summary

• Symbol: Rr27^tm1Tilg
• Name: regulatory region 27; targeted mutation 1, Shirley M Tilghman
• MGI ID: MGI:2451308
• Synonyms: H19^tm3Tilg
• Gene: Rr27 Location: Chr7:142133885-142136283 bp Genetic Position: Chr7, Syntenic
• Alliance: Rr27^tm1Tilg page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:81888
• Parent Cell Line: CJ7 (ES Cell)
• Strain of Origin: 129S1/Sv-Oca2⁺ Tyr⁺ Kitl⁺

Mutation description

• Allele Type: Targeted (Modified regulatory region)
• Mutation: Nucleotide substitutions
• Mutation details: Four point point mutations were inserted into the imprinting control region (ICR) upstream of the H19 transcription start site. The mutations were introduced at the Ctcf binding sites within the ICR. Single loxP sites were left flanking the ICR. RNase protection assays showed reduced levels of transcript in various tissues obtained from heterozygous females. (J:81888)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rr27 Mutation: 7 strains or lines available

References

• Original: J:81888 Schoenherr CJ, et al., CTCF maintains differential methylation at the Igf2/H19 locus. Nat Genet. 2003 Jan;33(1):66-9
• All: 1 reference(s)