Lifr^tm1Cgre
Targeted Allele Detail

Summary

• Symbol: Lifr^tm1Cgre
• Name: LIF receptor alpha; targeted mutation 1, Centre for Genome Research
• MGI ID: MGI:2651561
• Synonyms: LIFRbeta
• Gene: Lifr Location: Chr15:7120095-7226970 bp, + strand Genetic Position: Chr15, 3.46 cM
• Alliance: Lifr^tm1Cgre page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:82048
• Parent Cell Line: CGR8 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Approximately 20 kb of endogenous sequence was replaced with an IRES-beta-geo cassette. The deleted sequence contained 7 exons encoding the cytokine receptor domains (amino acids 85 through 444). The expression pattern of the beta-galactosidase gene recapitulated that of the endogenous gene. (J:82048)

Phenotypes

Key:

hm	homozygous	ht	heterozygous	tg	involves transgenes	√	phenotype observed
cn	conditional genotype	cx	complex: > 1 genome feature	ot	other: hemizygous, indeterminate,...	N	normal phenotype

Genotype/
Background:

	Allelic Composition	Genetic Background	Cell Line(s)
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hm1	Lifr^tm1Cgre/Lifr^tm1Cgre	either: (involves: 129P2/OlaHsd * CBA) or (involves: 129P2/OlaHsd * MF1)

Phenotypes:

Affected Systems	hm1
show or hide all annotated terms
growth/size/body	√
decreased body weight	√
mortality/aging	√
neonatal lethality, complete penetrance	√
nervous system	√
decreased motor neuron number	√

View phenotypes and curated references for all genotypes (concatenated display).

Expression

In Mice Carrying this Mutation:	18 assay results
In Structures Affected by this Mutation:	1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Lifr Mutation: 59 strains or lines available

References

• Original: J:82048 Li M, et al., Essential function of LIF receptor in motor neurons. Nature. 1995 Dec 14;378(6558):724-7
• All: 2 reference(s)