Krt10^tm1Der
Targeted Allele Detail

Summary

• Symbol: Krt10^tm1Der
• Name: keratin 10; targeted mutation 1, Dennis R Roop
• MGI ID: MGI:2652788
• Synonyms: mut^neo
• Gene: Krt10 Location: Chr11:99276080-99280190 bp, - strand Genetic Position: Chr11, 62.92 cM
• Alliance: Krt10^tm1Der page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:82300
• Parent Cell Line: AB2.2 (ES Cell)
• Strain of Origin: 129S7/SvEvBrd-Hprt1^b-m2

Mutation description

• Allele Type: Targeted (Humanized sequence)
• Mutation: Nucleotide substitutions
• Mutation details: An arginine to cysteine mutation was introduced to codon 154 (R154C) of exon 1 along with a floxed neo cassette via homologous recombination. R154C is a mutation commonly found in human epidermolytic hyperkeratosis (EHK). The floxed neo cassette prevents expression of the mutant allele until its removal by cre-mediated recombination. RNA analysis revealed the mutant allele is expressed at 35%-40% of wild-type. (J:82300)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Krt10 Mutation: 32 strains or lines available

References

• Original: J:82300 Arin MJ, et al., Focal activation of a mutant allele defines the role of stem cells in mosaic skin disorders. J Cell Biol. 2001 Feb 5;152(3):645-9
• All: 2 reference(s)