Bsn^tm1Gund
Targeted Allele Detail

Summary

• Symbol: Bsn^tm1Gund
• Name: bassoon; targeted mutation 1, Eckart D Gundelfinger
• MGI ID: MGI:2652946
• Synonyms: Bsn^-, Bsn^DeltaEX4/5
• Gene: Bsn Location: Chr9:107973221-108067583 bp, - strand Genetic Position: Chr9, 59.07 cM, cytoband F
• Alliance: Bsn^tm1Gund page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:82241
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A portion of exon 4 and all of exon 5 were replaced with a lacZ-neo cassette inserted by homologous recombination. Northern blot and RT-PCR analysis identified two transcripts produced by the mutant allele. A shorter transcript encoded a protein in which the amino terminal of the endogenous protein was adjoined to lacZ. A longer transcript was the result of the aberrant splicing of exons 3 and 6. Protein produced from the longer transcript was detected by Western blot analysis and lacked a central region that included the carboxy terminal half of the second zinc finger (aa 505 to 2889). (J:82241)

Disease models

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Bsn Mutation: 145 strains or lines available

References

• Original: J:82241 Altrock WD, et al., Functional inactivation of a fraction of excitatory synapses in mice deficient for the active zone protein bassoon. Neuron. 2003 Mar 6;37(5):787-800
• All: 25 reference(s)