Rr133^tm1Eno
Targeted Allele Detail

Summary

• Symbol: Rr133^tm1Eno
• Name: regulatory region 133; targeted mutation 1, Eric N Olson
• MGI ID: MGI:2653307
• Synonyms: BAenh-, Delta neoBAenh, Hand2^BA, Hand2^tm1Eno
• Gene: Rr133 Location: unknown Genetic Position: Chr8, Syntenic
• Alliance: Rr133^tm1Eno page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:81764
• Parent Cell Line: SM1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Modified regulatory region, Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: A 754 bp region, containing the 208 bp branchial arch enhancer upstream of the Hand2 gene, was replaced with a loxP site flanked neomycin resistance gene cassette, which was removed through subsequent cre-mediated recombination. Whole mount in situ hybridization of homozygous mutant embryos showed a lack of endogenous transcript in the ventrolateral portions of the first and second branchial arches. (J:81764)

Expression

In Mice Carrying this Mutation:	4 assay results
In Structures Affected by this Mutation:	13 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rr133 Mutation: 0 strains or lines available

References

• Original: J:81764 Yanagisawa H, et al., Targeted deletion of a branchial arch-specific enhancer reveals a role of dHAND in craniofacial development. Development. 2003 Mar;130(6):1069-78
• All: 7 reference(s)