Ptprj^tm1Taka
Targeted Allele Detail

Summary

• Symbol: Ptprj^tm1Taka
• Name: protein tyrosine phosphatase receptor type J; targeted mutation 1, Takamune Takahashi
• MGI ID: MGI:2653313
• Synonyms: CD148^DeltaCyGFP
• Gene: Ptprj Location: Chr2:90260098-90410939 bp, - strand Genetic Position: Chr2, 50.19 cM, cytoband E1-2
• Alliance: Ptprj^tm1Taka page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:82356
• Parent Cell Line: TL1/TL-1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Disruption caused by insertion of vector
• Mutation details: A targeting vector designed to replace sequence encoding 117 carboxy terminal amino acids with sequence encoding EGFP and neo was inserted at the endogenous locus via homologous recombination. RT-PCR and Northern blot analyses confirmed the presence of fusion transcript in mutant mice. Western blot analysis showed the level of fusion protein to be approximately half of that of the wild-type protein, indicating reduced stability. GFP fluorescence was not detected in mutant mice. (J:82356)

Expression

In Structures Affected by this Mutation:

11 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Ptprj Mutation: 71 strains or lines available

References

• Original: J:82356 Takahashi T, et al., A mutant receptor tyrosine phosphatase, CD148, causes defects in vascular development. Mol Cell Biol. 2003 Mar;23(5):1817-31
• All: 1 reference(s)