Tnf^tm1Sek
Targeted Allele Detail

Summary

• Symbol: Tnf^tm1Sek
• Name: tumor necrosis factor; targeted mutation 1, Kenji Sekikawa
• MGI ID: MGI:2653694
• Synonyms: TNF-, TNF-alpha-, TNFalpha^-, TNFalpha KO
• Gene: Tnf Location: Chr17:35418357-35420983 bp, - strand Genetic Position: Chr17, 18.59 cM
• Alliance: Tnf^tm1Sek page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:44841
• Parent Cell Line: TT2 (ES Cell)
• Strain of Origin: (C57BL/6NCrlj x CBA/JNCrlj)F1

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: All of exon 3 and a portion of exon 4 were replaced with a neomycin selection cassette inserted by homologous recombination. The deleted region encoded the DNA-binding domain (aa 84 through 174). (J:44841, J:92002)

Expression

In Structures Affected by this Mutation:

8 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Tnf Mutation: 48 strains or lines available

References

• Original: J:44841 Taniguchi T, et al., Failure of germinal center formation and impairment of response to endotoxin in tumor necrosis factor alpha-deficient mice. Lab Invest. 1997 Dec;77(6):647-58
• All: 68 reference(s)