Brca2^tm2Hst
Targeted Allele Detail

Summary

• Symbol: Brca2^tm2Hst
• Name: breast cancer 2, early onset; targeted mutation 2, Paul Hasty
• MGI ID: MGI:2653914
• Synonyms: brca2^lex2
• Gene: Brca2 Location: Chr5:150446095-150493794 bp, + strand Genetic Position: Chr5, 89.52 cM
• Alliance: Brca2^tm2Hst page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:82434
• Parent Cell Line: AB2.2 (ES Cell)
• Strain of Origin: 129S7/SvEvBrd-Hprt1^b-m2

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Most of exon 26 and all of exon 27 (coding nt 9265 through 9984) were replaced by a neomycin selection cassette inserted by homologous recombination. The deleted region included the RAD51-interacting domain. (J:49518, J:82434)

Tumor Data

List all tumor models in MMHCdb carrying Brca2^tm2Hst

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Brca2 Mutation: 132 strains or lines available

References

• Original: J:82434 Donoho G, et al., Deletion of Brca2 exon 27 causes hypersensitivity to DNA crosslinks, chromosomal instability, and reduced life span in mice. Genes Chromosomes Cancer. 2003 Apr;36(4):317-31
• All: 3 reference(s)