Ppp1r3a^tm1Adpr
Targeted Allele Detail

Summary

• Symbol: Ppp1r3a^tm1Adpr
• Name: protein phosphatase 1, regulatory subunit 3A; targeted mutation 1, Anna A DePaoli-Roach
• MGI ID: MGI:2653989
• Synonyms: RGL null
• Gene: Ppp1r3a Location: Chr6:14713976-14755273 bp, - strand Genetic Position: Chr6, 6.31 cM, cytoband A2
• Alliance: Ppp1r3a^tm1Adpr page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:68370
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129/Sv

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exon 1, which encodes the first 262 amino acids, was replaced with a neomycin selection cassette inserted by homologous recombination. The deleted region included the sequence encoding the C1 and glycogen binding domains as well as the Ser48 and Ser67 phosphorylation sites. The absence of normal protein was shown by Western blot analysis of homozygous skeletal and cardiac muscle. (J:68370)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ppp1r3a Mutation: 58 strains or lines available

Notes

Either CCE, E14, or R1 ES cells, all derived from a 129 background, were used.

References

• Original: J:68370 Suzuki Y, et al., Insulin control of glycogen metabolism in knockout mice lacking the muscle-specific protein phosphatase PP1G/RGL. Mol Cell Biol. 2001 Apr;21(8):2683-94
• All: 4 reference(s)