Cav1^tm1Tt
Targeted Allele Detail

Summary

• Symbol: Cav1^tm1Tt
• Name: caveolin 1, caveolae protein; targeted mutation 1, Timothy C Thompson
• MGI ID: MGI:2654177
• Synonyms: cav-1-, Cav1-
• Gene: Cav1 Location: Chr6:17306387-17341323 bp, + strand Genetic Position: Chr6, 7.73 cM, cytoband A2
• Alliance: Cav1^tm1Tt page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:82490
• Parent Cell Line: AB2.2 (ES Cell)
• Strain of Origin: 129S7/SvEvBrd-Hprt1^b-m2

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A floxed neo cassette and loxP site were introduced flanking exon 2 via homologous recombination. Transient expression of cre-recombinase in correctly targeted ES cells resulted in deletion of exon 2 and the neo cassette leaving behind a single loxP site. Absence of protein product in homozygous mutant animals was confirmed by Western blot anlysis of heart, spleen, and lung extracts. (J:82490)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cav1 Mutation: 31 strains or lines available

References

• Original: J:82490 Cao G, et al., Disruption of the caveolin-1 gene impairs renal calcium reabsorption and leads to hypercalciuria and urolithiasis. Am J Pathol. 2003 Apr;162(4):1241-8
• All: 7 reference(s)