Kcnmb1^tm1Pong
Targeted Allele Detail

Summary

• Symbol: Kcnmb1^tm1Pong
• Name: potassium large conductance calcium-activated channel, subfamily M, beta member 1; targeted mutation 1, Olaf Pongs
• MGI ID: MGI:2654278
• Synonyms: BKbeta1^-
• Gene: Kcnmb1 Location: Chr11:33913013-33923641 bp, + strand Genetic Position: Chr11, 19.32 cM
• Alliance: Kcnmb1^tm1Pong page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:82471
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: Gene targeting was used to flank exon 1 with a floxed PGK-neo cassette on one side and a loxP site on the other side. Correctly targeted ES cells were transiently transfected with a cre-recombinase plasmid to delete exon 1. Absence of gene expression in homozygous mutant animals was confirmed by Northern blot and RT-PCR analysis. (J:82471)

Expression

In Structures Affected by this Mutation:

5 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Kcnmb1 Mutation: 16 strains or lines available

References

• Original: J:82471 Pluger S, et al., Mice with disrupted BK channel beta1 subunit gene feature abnormal Ca(2+) spark/STOC coupling and elevated blood pressure. Circ Res. 2000 Nov 24;87(11):E53-60
• All: 3 reference(s)