Wt1^tm1Hst
Targeted Allele Detail

Summary

• Symbol: Wt1^tm1Hst
• Name: WT1 transcription factor; targeted mutation 1, Nicholas D Hastie
• MGI ID: MGI:2654351
• Gene: Wt1 Location: Chr2:104956874-105003959 bp, + strand Genetic Position: Chr2, 55.06 cM, cytoband E
• Alliance: Wt1^tm1Hst page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:82515
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Modified isoform(s), Null/knockout)
• Mutations: Insertion, Single point mutation
• Mutation details: A nonsense mutation was introduced into exon 1, 12 codons downstream of the CUG start codon and upstream of the AUG start codon. A floxed hygro cassette was excised in vitro, leaving a single loxP site in a nonconserved region within in intron 1. Western blot analysis of homozygous mutant fetal kidney and testis tissue showed that while the CUG-initiated isoforms were undetected, normal levels of AUG-initiated isoforms were present. (J:82515)

Expression

In Mice Carrying this Mutation:

3 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Wt1 Mutation: 34 strains or lines available

References

• Original: J:82515 Miles CG, et al., Mice lacking the 68-amino-acid, mammal-specific N-terminal extension of WT1 develop normally and are fertile. Mol Cell Biol. 2003 Apr;23(7):2608-13
• All: 2 reference(s)