dblr
Transgenic Allele Detail

Summary

• Symbol: dblr
• Name: doubleridge
• MGI ID: MGI:2654910
• Synonyms: Dkk1^d, T3
• Gene: dblr Location: Chr19:26305715-37234461 bp Genetic Position: Chr19, cytoband C
• Alliance: dblr page

Transgene origin

• Strain of Origin: (C57BL/6J x SJL/J)F2

Transgene description

• Transgene Type: Transgenic
• Mutations: Insertion, Intragenic deletion
• Mutation details: The doubleridge mutant was identified in a phenotype screen of a set of transgenic mouse lines for recessive insertional mutants. The mutation resulted from random insertion of a 50-kb tandem transgene cluster accompanied by deletion of a 60 kb DNA segment between and downstream (3') of two oppositely transcribed genes: Mbl2 and Dkk1, at respective distances of 90 kb and 150 kb. The insertion/deletion occurred in a chromosomal segment derived from the SJL parent of the microinjected hybrid zygote. The transgene is not expressed. In heterozygous mutant mice, significantly lower levels of Dkk1 mRNA are expressed from the chromosome bearing the mutation than from the wild-type chromosome. Transcript levels do not differ between mutant and non-mutant chromosomes for Mbl2 or Prkg1, which lies upstream of Mbl2. (J:91047)
• Inheritance: Recessive

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any dblr Mutation: 0 strains or lines available

References

• Original: J:82633 Adamska M, et al., Doubleridge, a mouse mutant with defective compaction of the apical ectodermal ridge and normal dorsal-ventral patterning of the limb. Dev Biol. 2003 Mar 15;255(2):350-62
• All: 9 reference(s)