Aqp5^tm1Amn
Targeted Allele Detail

Summary

• Symbol: Aqp5^tm1Amn
• Name: aquaporin 5; targeted mutation 1, Anil G Menon
• MGI ID: MGI:2655164
• Synonyms: Aqp5^-
• Gene: Aqp5 Location: Chr15:99488909-99492710 bp, + strand Genetic Position: Chr15, 56.13 cM
• Alliance: Aqp5^tm1Amn page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:82588
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129X1/SvJ

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A 600 bp fragment encompassing exon 3 and surrounding intronic sequence was replaced with a neomycin selection cassette inserted by homologous recombination. The deleted region encoded extracellular loop E, which contains the conserved Asn-Pro-Ala motif and the merucury-sensitive cysteine at position 182. Neither transcript nor protein was by analyses of salivary glands obtained from homozygous mutant mice. (J:82588)

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Aqp5 Mutation: 19 strains or lines available

References

• Original: J:82588 Krane CM, et al., Salivary acinar cells from aquaporin 5-deficient mice have decreased membrane water permeability and altered cell volume regulation. J Biol Chem. 2001 Jun 29;276(26):23413-20
• All: 11 reference(s)