Rr27^tm1Kpfe
Targeted Allele Detail

Summary

• Symbol: Rr27^tm1Kpfe
• Name: regulatory region 27; targeted mutation 1, Karl Pfeifer
• MGI ID: MGI:2655218
• Synonyms: DMR^flox, H19^tm1Kpfe
• Gene: Rr27 Location: Chr7:142133885-142136283 bp Genetic Position: Chr7, Syntenic
• Alliance: Rr27^tm1Kpfe page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:82798
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Conditional ready, Modified regulatory region, No functional change)
• Mutation: Insertion
• Mutation details: The differentially methylated region (DMR), located upstream of the H19 transcription initiation site, was left flanked by single loxP sites at -6.5 to -0.8 kb upstream of H19 major start within the regulatory region 27 (Rr27) and a neomycin-resistance gene flanked by loxP sites inserted at the -7.0kb upstream of H19. The neo-cassette was removed in ES cells by transiently transfecting with a Cre recombinase-expressing plasmid. (J:82798)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Rr27 Mutation: 7 strains or lines available

References

• Original: J:82798 Srivastava M, et al., H19 and Igf2 monoallelic expression is regulated in two distinct ways by a shared cis acting regulatory region upstream of H19. Genes Dev. 2000 May 15;14(10):1186-95
• All: 6 reference(s)