Tg(Hbb-b1)83Clo
Transgene Detail

Summary

• Symbol: Tg(Hbb-b1)83Clo
• Name: transgene insertion 83, Cecilia W Lo
• MGI ID: MGI:2655271
• Synonyms: TgN(Hbb-b1)83Clo, TgN(Hbb-b1)83Lo
• Transgene: Tg(Hbb-b1)83Clo Location: unknown Genetic Position: Chr3, Syntenic
• Alliance: Tg(Hbb-b1)83Clo page

Transgene origin

• Strain of Origin: C57BL/6J or SJL/J

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(Hbb-b1)83Clo expresses 1 gene
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  mouse	Hbb-b1 (MGI:96021)

• Mutation details: The transgenic construct comprised a pBR322-derived plasmid containing a 7.0-kb mouse genomic EcoRI fragment that included the mouse beta-major globin gene. In situ hybridization of a plasmid-derived probe to bone marrow metaphase chromosome spreads localized the integration site to the telomeric end of Chr 3. 800-1000 tandem copies of the transgene were estimated, by Southern blot analysis, to have integrated in this transgenic line. Northern blot analysis confirmed the expected lack (due to presence of the pBR322 sequences) of transcription of the exogenous globin genes, and analysis of blood from mice bearing the transgene detected no expression of the beta-major globin chain in their hemoglobin. (J:82632, J:123337)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 4 strains available Cell Lines: 0 lines available

Notes

Origin Note: To generate this transgene, the authors "ionotophoretically injected the plasmid...into the pronuclei of mouse eggs (C57BL/6J x SJL/J)." (J:123337)

References

• Original: J:90003 Lo CW, Localization of low abundance DNA sequences in tissue sections by in situ hybridization. J Cell Sci. 1986 Mar;81:143-62
• All: 13 reference(s)