Chrm1^tm1Jwe
Targeted Allele Detail

Summary

• Symbol: Chrm1^tm1Jwe
• Name: cholinergic receptor, muscarinic 1, CNS; targeted mutation 1, Jurgen Wess
• MGI ID: MGI:2655332
• Synonyms: M1 -, M1R^-, mAChR1^-
• Gene: Chrm1 Location: Chr19:8641369-8660970 bp, + strand Genetic Position: Chr19, 5.44 cM
• Alliance: Chrm1^tm1Jwe page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:74588
• Parent Cell Line: TC1/TC-1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A PGK-neomycin resistance cassette replaced a 0.28 kb genomic fragment that contained the translation start site and the region coding for the first 54 amino acids of the encoded protein. Immunoprecipitation studies demonstrated that no detectable protein was present in membranes prepared from hippocampus of homozygous mice. (J:74588)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Chrm1 Mutation: 27 strains or lines available

References

• Original: J:74588 Fisahn A, et al., Muscarinic induction of hippocampal gamma oscillations requires coupling of the M1 receptor to two mixed cation currents. Neuron. 2002 Feb 14;33(4):615-24
• All: 40 reference(s)