Fn1^tm1Feb
Targeted Allele Detail

Summary

• Symbol: Fn1^tm1Feb
• Name: fibronectin 1; targeted mutation 1, Francisco E Baralle
• MGI ID: MGI:2655527
• Synonyms: EDA^fl
• Gene: Fn1 Location: Chr1:71624679-71692359 bp, - strand Genetic Position: Chr1, 36.05 cM, cytoband C1-C5
• Alliance: Fn1^tm1Feb page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:84274
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129X1/SvJ

Mutation description

• Allele Type: Targeted (Conditional ready, Modified isoform(s))
• Mutation: Nucleotide substitutions
• Mutation details: The endogenous EDA exon, which is subject to alternative splicing, was replaced with a floxed EDA exon modified with point mutations for optimization of both the 5' and the 3' splice sites. RNase protection and RT-PCR analysis confirmed constitutive EDA exon inclusion, showing the EDA exon to be included in 99-100% of transcript in all tissues of homozygous mutant mice. (J:84274)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Fn1 Mutation: 130 strains or lines available

References

• Original: J:84274 Muro AF, et al., Regulated splicing of the fibronectin EDA exon is essential for proper skin wound healing and normal lifespan. J Cell Biol. 2003 Jul 7;162(1):149-60
• All: 10 reference(s)