Pafah1b2^tm1Arai
Targeted Allele Detail

Summary

• Symbol: Pafah1b2^tm1Arai
• Name: platelet-activating factor acetylhydrolase, isoform 1b, subunit 2; targeted mutation 1, Hiroyuki Arai
• MGI ID: MGI:2655809
• Synonyms: alpha2^-
• Gene: Pafah1b2 Location: Chr9:45876609-45923988 bp, - strand Genetic Position: Chr9, 25.36 cM
• Alliance: Pafah1b2^tm1Arai page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:82792
• Parent Cell Line: RW-4 (ES Cell)
• Strain of Origin: 129X1/SvJ

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: All of exon 2 and the majority of exon 3 were replaced with a neomycin selection cassette inserted by homologous recombination. The deleted region included the start codon and the catalytic serine residue. Protein was undetected by Western blot analysis of homozygous mutant mice. (J:82792)

Expression

In Mice Carrying this Mutation:	19 assay results
In Structures Affected by this Mutation:	1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Pafah1b2 Mutation: 20 strains or lines available

References

• Original: J:82792 Koizumi H, et al., Targeted disruption of intracellular type I platelet activating factor-acetylhydrolase catalytic subunits causes severe impairment in spermatogenesis. J Biol Chem. 2003 Apr 4;278(14):12489-94
• All: 3 reference(s)