Pdgfra^tm5Sor
Targeted Allele Detail

Summary

• Symbol: Pdgfra^tm5Sor
• Name: platelet derived growth factor receptor, alpha polypeptide; targeted mutation 5, Philippe Soriano
• MGI ID: MGI:2656146
• Synonyms: alpha^PI3K, Pdgfralpha^PI3K
• Gene: Pdgfra Location: Chr5:75312953-75358876 bp, + strand Genetic Position: Chr5, 39.55 cM
• Alliance: Pdgfra^tm5Sor page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:73817
• Parent Cell Line: AK7 (ES Cell)
• Strain of Origin: 129S4/SvJaeSor

Mutation description

• Allele Type: Targeted (Hypomorph, Inserted expressed sequence)
• Mutation: Insertion
• Mutation details: A cDNA encoding a mutated receptor consisting of point mutations encoding the tyrosine 731/742 sites was targeted into the Pdgfra locus with a loxP-flanked neomycin cassette. Western blot analysis indicated that expression of the targeted Pdgfra locus was disrupted and the mutant receptor was expressed. (J:73817)

Expression

In Mice Carrying this Mutation:

2 assay results 1 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Pdgfra Mutation: 88 strains or lines available

References

• Original: J:73817 Klinghoffer RA, et al., An allelic series at the PDGFalphaR locus indicates unequal contributions of distinct signaling pathways during development. Dev Cell. 2002 Jan;2(1):103-13
• All: 10 reference(s)