Lgth6^MRL/MpJ
QTL Variant Detail

Summary

• QTL variant: Lgth6^MRL/MpJ
• Name: body length 6; MRL/MpJ
• MGI ID: MGI:2656865
• QTL: Lgth6 Location: Chr11:83733420-83733652 bp Genetic Position: Chr11, cM position of peak correlated region/allele: 51.23 cM
• QTL Note: genome coordinates based on the marker associated with the peak LOD score

Variant origin

• Strain of Specimen: MRL/MpJ

Variant description

• Allele Type: QTL
• Mutation: Undefined
• This allele confers increased fat mass in females compared to SJL/J. (J:106033)
• Inheritance: Other (see notes)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Notes

Lgth6 exhibits additive inheritance.

Mapping and Phenotype information for this QTL, its variants and associated markers

J:81606

Genome scan was performed on 633 (MRL/MpJ x SJL/J)F2 animals to identify QTLs associated with lean body mass and body length. Parental strain MRL/MpJ exhibits a higher amount of lean body mass compared to parental strain SJL/J. Several lean body mass (Lbm) and body length (Lgth) QTLs were mapped. MRL/MpJ-derived alleles confer increased lean body mass at all Lbm loci. Lbm1, Lbm2, Lgth1, and Lgth2 map to mouse Chromosome 2 at 26.3 cM (LOD=3.6) , 50.3 cM (LOD=9.0), 50.3 cM (LOD=10.8), and 72.1 cM (LOD=9.7), respectively. (Lbm2 and Lgth1 overlap.) Lbm1 is inherited additively while Lbm2 is inherited dominantly. Lgth3 maps to 30.6 cM on mouse Chromosome 4 with LOD=2.7 at D4Mit178. Lbm3 and Lbm4 map to mouse Chromosome 6 at 17.5 cM (LOD=4.7) and 49.2 cM (LOD=4.7), respectively. Lbm3 exhibits a dominant mode of inheritance and Lbm4 exhibits an additive mode of inheritance. Lbm5 maps to 8 cM on mouse Chromosome 7 with LOD=2.9 at D7Mit22 and exhibits an additive mode of inheritance. Lbm6 and Lgth4 both map to 7.7 cM (LOD=7.5 and LOD=4.7, respectively), and Lbm7 and Lgth5 both map to 31.7 cM (LOD=4.7 and LOD=2.3, respectively) on mouse Chromosome 9. Lbm6 and Lbm7 exhibit dominant inheritance. Lgth6 maps to 43.7 cM on mouse Chromosome 11 with LOD=4.8 at D11Mit36. Lbm8and Lgth8 both map to 23 cM (LOD=4.2 and LOD=2.3, respectively), and Lbm9 and Lgth7 both map to 2.2 cM (LOD=3.9 and LOD=2.7, respectively) on mouse Chromosome 13. Lbm8 and Lbm9 exhibit additive inheritance. Lbm10 maps to 40.4 cM on mouse Chromosome 14with LOD=4.7 at D14Mit32 and exhibits additive inheritance.

J:81605

Genome scan was performed on 633 animals from an (MRL/MpJ x SJL/J)F2 cross to identify loci influencing muscle size and body length. 119 markers were screened at an average resolution of 15 cM. Five QTLs showing significant linkage to muscle size (P<0.005) were mapped. Musz1 maps to 5.5 cM on mouse Chromosome 7, Musz2 and Musz3 map to 21.9 cM and 40.4 cM on mouse Chromosome 14, respectively, Musz4 maps to 13.1 cM on mouse Chromosome 15, and Musz5 maps to 41.5 cM on mouse Chromosome 17. These 5 QTLs explain 19.2% of the phenotypic variance. A sixth suggestive QTL mapped to 7.7 cM on mouse Chromosome 9 and appears to influence body length as well as muscle size.

Previously identified body length QTLs were detected in this study: Lgth1 (LOD=6.21 at D2Mit182) and Lgth2 (LOD=8.7 at D2Mit304) on mouse Chromosome 2, Lgth4 (LOD=4.1 at D9Mit90) on mouse Chromosome 9, and Lgth6 (LOD=2.7 at D11Mit36) on mouse Chromosome 11.

A novel body length QTL, Lgth9, mapped to 6.6 cM on mouse Chromosome 17 with LOD=4.1 at D17Mit175.

J:106033

Linkage analysis was performed on 621 female mice from a (MRL/MpJ x SJL/J)F2 intercross to identify QTLs associated with obesity and serum lipid levels. Parental strain MRL/MpJ displays increased body weight, fat mass, serum cholesterol, and serum HDL, and decreased serum triglycerides compared to parental strain SJL/J. Female F2 mice were phenotyped at 7 weeks of age and genotyped at 132 polymorphic loci.

Significant linkage to serum cholesterol and HDL mapped to 95 cM on mouse Chromosome 1 near D1Mit453. This locus is named Chldq1 (cholesterol and HDL QTL 1). MRL/MpJ-derived alleles at Chdlq1 confer increased serum cholesterol and HDL with additive inheritance. Tbbmd1 (110 cM) is a femur bone mineral density QTL mapping near Chldq1, and Apoa2 at 92.6cM is a potential candidate gene. Linkage to serum triglycerides mapped to 76.2 cM near D1Mit43 (LOD=3.8) and is named Tglq1 (triglyceride QTL 1). This locus explains 3.4% of the serum triglyceride variance in females. MRL/MpJ-derived alleles at Tglq1 confer increased serum triglyceride levels with dominant inheritance.

Significant linkage to serum cholesterol (LOD=6.61) and HDL (LOD=7.02) mapped to 40 cM on mouse Chromosome 3 near D3Mit217. This locus is named Chldq2 (cholesterol and HDL QTL 2). MRL/MpJ-derived alleles at Chldq2 confer increased serum cholesterol and HDL with additive inheritance.

Significant linkage to serum cholesterol (LOD=3.32) and HDL (LOD=4.86) mapped to 45 cM - 55 cM on mouse Chromosome 5 near D5Mit239. This locus, Chldq3 (cholesterol and HDL QTL 3), also exhibits suggestive linkage to triglycerides at 55 cM (LOD=3.05). MRL/MpJ-derived alleles at Chldq3 confer increased serum cholesterol and HDL with additive inheritance.

Suggestive linkage to fat mass mapped to 50 cM on mouse Chromosome 7 (LOD=2.42). Significant linkage to non-HDL cholesterol mapped to 15 cM with LOD=3.69. This locus, Chldq4 (cholesterol and HDL QTL 4), also exhibits linkage to serum cholesterol (LOD=4.74) and HDL (LOD=2.95) at 10 cM. MRL/MpJ-derived allelesat Chldq4 confer increased serum cholesterol and HDL with additive inheritance.

Suggestive linkage to non-HDL cholesterol mapped to 40 cM on mouse Chromosome 9 with LOD=2.73.

Linkage to fat mass mapped to 43 cM on mouse Chromosome 11 near D11Mit36 (LOD=11.51). This locus is the previously identified QTL Lgth6 (body length 6). Lgth6 explains 12% of the variance for female fat mass. MRL/MpJ-derived alleles at Lgth6 confer increased fat mass with additive inheritance. Potential candidate genes for Lgth6 include Nos2 (45.2 cM), Hcrt (61.2 cM), Alox12 (40 cM), Alox3, Alox15 (40 cM), Alox12b (37 cM), and Alox12e (40 cM).

Linkage to non-HDL cholesterol mapped to 5 cM on mouse Chromosome 12 with LOD=5.09. This locus explains 6.2% of the variance and is named Nhq1 (non-HDL cholesterol QTL 1). Linkage to serum triglycerides mapped to 26 cM near D12Mit201 (LOD=4.1). This locus explains 4.6% of the variance and is named Tglq2 (triglyceride QTL 2). SJL/J-derived alleles at Tglq2 confer increased serum triglyceridesin female animals with an additive mode of inheritance. Mwq4 (20 cM), Swq2 (22 cM), and Afw10 (21 cM) are previously identified obesity QTLs mapping near Tglq2.

Suggestive linkage to serum cholesterol (LOD=2.62) and HDL (LOD=2.43) mapped to 40 cM -45 cMon mouse Chromosome 14. This locus explains 2.9% of the cholesterol variance and 2.6% of the HDL variance in females.

On mouse Chromosome 16, significant linkage to fat mass mapped to 50 cM near D16Mit51 (LOD=3.99). This locus explains 3.6% of thevarianceand is named Ftms1 (fat mass 1). SJL/J-derived alleles at Ftms1 confer increased fat mass with recessive inheritance. Liph (14.8 cM) is a potential candidate gene for Ftms1.

Linkage to serum cholesterol mapped to 25 cM on mouse Chromosome 17 with LOD=3.73). This locus, Chldq5 (cholesterol and HDL QTL 5), also exhibits suggestive linkage to HDL cholesterol (LOD=2.5) and triglycerides (LOD=2.59).

References

• Original: J:81606 Masinde GL, et al., Quantitative trait loci (QTL) for lean body mass and body length in MRL/MPJ and SJL/J F(2) mice. Funct Integr Genomics. 2002 Aug;2(3):98-104
• All: 3 reference(s)