Kif5a^tm1Gsn
Targeted Allele Detail

Summary

• Symbol: Kif5a^tm1Gsn
• Name: kinesin family member 5A; targeted mutation 1, Lawrence S B Goldstein
• MGI ID: MGI:2656900
• Synonyms: KIF5A^null, Kinesin-1A^-
• Gene: Kif5a Location: Chr10:127061565-127099217 bp, - strand Genetic Position: Chr10, 74.5 cM
• Alliance: Kif5a^tm1Gsn page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:82911
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Two coding exons were flanked with a floxed PGK-neo cassette and single loxP site via homologous recombination. The 2 exons and PGK-neo cassette were removed by transient expression of cre recombinase in correctly targeted ES cells. Western blot analysis of brain extracts from homozygous mutant embryos confirmed the absence of gene expression. (J:82911)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Kif5a Mutation: 55 strains or lines available

References

• Original: J:82911 Xia CH, et al., Abnormal neurofilament transport caused by targeted disruption of neuronal kinesin heavy chain KIF5A. J Cell Biol. 2003 Apr 14;161(1):55-66
• All: 4 reference(s)