Atp2a1^tm1Dhm
Targeted Allele Detail

Summary

• Symbol: Atp2a1^tm1Dhm
• Name: ATPase, Ca++ transporting, cardiac muscle, fast twitch 1; targeted mutation 1, David H MacLennon
• MGI ID: MGI:2656945
• Synonyms: SERCA1^-, SERCA1-null
• Gene: Atp2a1 Location: Chr7:126045032-126062245 bp, - strand Genetic Position: Chr7, 69.04 cM
• Alliance: Atp2a1^tm1Dhm page

Atp2a1^tm1Dhm/Atp2a1^tm1Dhm newborns are cyanotic

Show the 3 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:82947
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The gene was disrupted by replacement of exons 1-4 with a PGK-neo cassette via homologous recombination. Absence of gene expression in homozygous mutant animals was confirmed by Western blot and RT-PCR analysis. (J:82947)

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Atp2a1 Mutation: 45 strains or lines available

References

• Original: J:82947 Pan Y, et al., Targeted disruption of the ATP2A1 gene encoding the sarco(endo)plasmic reticulum Ca2+ ATPase isoform 1 (SERCA1) impairs diaphragm function and is lethal in neonatal mice. J Biol Chem. 2003 Apr 11;278(15):13367-75
• All: 1 reference(s)